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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.20more amino acid residue 192-1085 expressed as His-tagged protein in Escherichia coli BL21(DE3) Rosetta2 717834
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.20more synthesis and cloning of an optimized gene, with a codon adaptation index and an average G/C content higher than the native gene. Optimal expression is achieved by induction with 1 mM IPTG at 37°C for 18 h. This allows obtaining 100 mg of recombinant enzyme per liter of culture medium. The protein shows only limited signs of proteolytic degradation, and purification in a single step by immobilized metal ion affinity chromatography increases purity 27fold 732862
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