EC Number   |
Protein Variants |
Reference |
|---|
    3.2.2.5 | D133A |
site-directed mutagenesis |
731344 |
| 3.2.2.5 | D330G |
mutation of the inactive enzyme variant SPNH5 causes only a slight increase in activity, but the mutation in SPNJ4 causes an increase in activity |
-, 732106 |
| 3.2.2.5 | DELTA41-140 |
166% of wild-type activity, loss of translocation competence |
683896 |
| 3.2.2.5 | DELTA41-190 |
221% of wild-type activity, loss of translocation competence |
683896 |
| 3.2.2.5 | DELTA41-240 |
3.5% of wild-type activity, loss of translocation competence |
683896 |
| 3.2.2.5 | DELTA41-90 |
212% of wild-type activity, less stable than wild-type |
683896 |
| 3.2.2.5 | E124A |
site-directed mutagenesis, the mutant is not inhibited by cyanidin in contrast to the wild-type enzyme |
731344 |
| 3.2.2.5 | E179G |
site-directed mutagenesis |
709059 |
| 3.2.2.5 | E202A |
site-directed mutagenesis, the mutant is much less inhibited by cyanidin compared to the wild-type enzyme |
731344 |
| 3.2.2.5 | E226 |
crystallization data |
683631 |
