EC Number   |
Protein Variants |
Reference |
|---|
   3.2.2.21 | D112N |
site-directed mutagenesis |
646887 |
| 3.2.2.21 | D113N |
the mutation results in a 100fold decrease in the single-turnover rate of 7-methylguanine excision relative to the wild type enzyme |
699549 |
| 3.2.2.21 | D152N |
site-directed mutagenesis |
646887 |
| 3.2.2.21 | D238N |
inactive |
731306 |
| 3.2.2.21 | D238N |
site-directed mutagenesis |
646877 |
| 3.2.2.21 | D252N |
site-directed mutagenesis |
646887 |
| 3.2.2.21 | D259N |
site-directed mutagenesis |
646887 |
| 3.2.2.21 | D260N |
site-directed mutagenesis |
646887 |
| 3.2.2.21 | D310N |
site-directed mutagenesis |
646887 |
| 3.2.2.21 | more |
recombinant expression of enzyme and targeting to mitochondria or nucleus of primary astrocytes. Increasing enzyme activity significantly increases base excision repair kinetics in both the mitochondria and nuclei. Increased nuclear enzyme activity results in cell death in astrocyte cultures treated with methylnitrososurea |
680028 |
