EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.94 | D107A |
activity similar to wild-type enzyme |
655108 |
| 3.2.1.94 | D163A |
activity similar to wild-type enzyme |
655108 |
| 3.2.1.94 | D198N |
0.003% of wild-type activity, largely increased Kd-value |
664121 |
| 3.2.1.94 | D207A |
4fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 28fold decrease in kcat/Km for dextran T2000 as compared to wild-type value |
-, 751049 |
| 3.2.1.94 | D207A/N209A |
4.2fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 17.8fold decrease in kcat/Km for dextran T2000 as compared to wild-type value |
-, 751049 |
| 3.2.1.94 | D227A |
no activity of dextran hydrolysis |
655108 |
| 3.2.1.94 | D227E |
decreased activity of dextran hydrolysis |
655108 |
| 3.2.1.94 | D227N |
decreased activity of dextran hydrolysis |
655108 |
| 3.2.1.94 | D227Q |
decreased activity of dextran hydrolysis |
655108 |
| 3.2.1.94 | D266N |
0.011% of wild-type activity, without change in substrate binding ability |
664121 |
