EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.93 | D203E |
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside |
-, 749495 |
| 3.2.1.93 | D329E |
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside |
749495 |
| 3.2.1.93 | E254D |
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside |
-, 749495 |
| 3.2.1.93 | H281A |
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 5.3fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 5.25fold as compared to wild-type value |
-, 749495 |
| 3.2.1.93 | H282A |
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 2.65fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 2.6fold as compared to wild-type value |
749495 |
| 3.2.1.93 | K284A |
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 1.5fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 1.5fold as compared to wild-type value |
-, 749495 |
| 3.2.1.93 | K292A |
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 265fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 189fold as compared to wild-type value |
-, 749495 |
| 3.2.1.93 | more |
enzyme gene disruption mutant, unable to grow on trehalose, while trehalose uptake remains unaffected. Cryoprotective effect of trehalose is reduced |
-, 663716 |
