EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.85 | E160Q |
1000fold reduction in activity compared to wild type |
208852 |
| 3.2.1.85 | E170Q |
catalytic mutant |
-, 750522 |
| 3.2.1.85 | E375Q |
complete loss of activity |
208852 |
| 3.2.1.85 | G332R |
complete loss of catalytic activity, but same behavior during purification and in affinity to antisera as wild type |
208852 |
| 3.2.1.85 | K435V/Y437F |
significantly reduced substrate binding and turnover |
657216 |
| 3.2.1.85 | more |
construction of a disruption mutant of gene bglS, encoding phospho-beta-glucosidase, in phospho-beta-galactosidase-deficient strain IL1403, genetic organization and chromosomal localizations in mutant strain IL1403 showig the beta-galactosidase-negative phenotype |
663711 |
| 3.2.1.85 | more |
hybrid protein with protein A |
208851 |
| 3.2.1.85 | S428D/K435V/Y437F |
no activity |
657216 |
| 3.2.1.85 | W429A |
improved specificity for glucosides |
657216 |
| 3.2.1.85 | W433A |
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity |
-, 750522 |
