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EC Number
Protein Variants
Commentary
Reference
3.2.1.83
E168D
inactive
-
,
751098
3.2.1.83
more
construction of a chimeric fusion protein consisting of the catalytic domains of kappa-carrageenase CgkA and lambda-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain from Clostridium cellulovorans. The recombinant cCgkA and cCglA are assembled with scaffoldin miniCbpA from Clostridium cellulovorans via cohesin and dockerin interaction. Carbohydrate binding module in scaffoldin is used as a tag
732213
3.2.1.83
Q171A
the mutant shows 157% of wild type activity
-
,
751098
3.2.1.83
R151A
the mutant shows 199% of wild type activity
-
,
751098
3.2.1.83
R196A
the mutant shows 3% of wild type activity
-
,
751098
3.2.1.83
R260A
the mutant shows less than 1% of wild type activity
751098
3.2.1.83
R92A
the mutant shows 102% of wild type activity
-
,
751098
3.2.1.83
W266A
the mutant shows 5% of wild type activity
751098
Results
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