EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.70 | C129S/C532S |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme, the mutant predominantly catalyzes hydrolysis |
-, 732119 |
| 3.2.1.70 | D194A/C129S/C532S |
site-directed mutagenesis |
-, 732119 |
| 3.2.1.70 | D194C/C129S/C532S |
site-directed mutagenesis, the mutant shows highly reduced activity compared with the wild-type enzyme and mutant C129S/C532S, oxidation with KI activates the mutant by 330fold which is 0.27% activity of the activity of mutant C129S/C532S, the oxidized mutant Ox-D194C-2CS catalyzes both hydrolysis and transglucosylation |
-, 732119 |
| 3.2.1.70 | D318A |
shows an activity level identical to that of the wild-type |
693147 |
| 3.2.1.70 | E236Q |
catalysis is compromised in complex with isomaltotriose |
699532 |
| 3.2.1.70 | E458Q |
mutant enzyme shows 0.0005% of the wild-type activity |
-, 665766 |
| 3.2.1.70 | E656Q |
mutant enzyme shows 0.0003% of the wild-type activity |
-, 665766 |
| 3.2.1.70 | E94A |
shows a relatively minor change in the alpha-1,6-glucosidase activity, but a sharply increased alpha-1,4-transferase activity compared with the wild-type |
693147 |
| 3.2.1.70 | F262A |
the mutant enzyme has a stronger preference for transglucosylation than that of the wild type enzyme |
-, 750539 |
| 3.2.1.70 | F262W |
the mutant enzyme has a weaker preference for transglucosylation than that of the wild type enzyme |
-, 750539 |
