EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.142 | A885S |
single nucleotide polymorphism associated with thermostability |
709903 |
| 3.2.1.142 | D730R |
modest change in the association rate constant with endogenous inhibitor LDI. Residue D730 binds in a positively charged pocket on the surface of LDI via a hydrogen bond and a salt bridge to residue Arg34 and a hydrogen bond to Arg84 of LDI. D730 adopts different rotamers in the free enzyme and enzyme bound to LDI |
738645 |
| 3.2.1.142 | D730W |
modest change in the association rate constant with endogenous inhibitor LDI. Residue D730 binds in a positively charged pocket on the surface of LDI via a hydrogen bond and a salt bridge to residue Arg34 and a hydrogen bond to Arg84 of LDI. D730 adopts different rotamers in the free enzyme and enzyme bound to LDI |
738645 |
| 3.2.1.142 | E510A |
0.0004% residual activity with pullulan |
738874 |
| 3.2.1.142 | L102R/T233A/S235G/G298A/C415R/A885S/G888C |
amino acid substitutions identified by alignment of the limit dextrinase sequences of varieties Galleon and Maud |
709903 |
| 3.2.1.142 | M440G |
2.6fold decrease in catalytic efficiency |
738874 |
| 3.2.1.142 | more |
amino acids 362-370 of Ask are replaced with the corresponding sequence of type I pullulanase Pul-LM14-2 from Anoxybacillus sp. LM14-2. Unlike wild-type, the mutant enzyme forms reducing sugars on digesting starch |
-, 737518 |
| 3.2.1.142 | more |
investigation on genetic diversity of limit dextrinase using single strand conformation polymorphism based on the amino acid substitutions. Only limited genetic variation is detected in the current malting barley varieties, although wide variation is observed in the wider barley germplasm |
709903 |
| 3.2.1.142 | more |
investigation on genetic diversity of limit dextrinase using single strand conformation polymorphism based on the amino acid substitutions. Only limited genetic variation is detected in the current malting barley varieties, although wide variation is observed in the wider barley germplasm. No polymorphism is associated with the enzyme activity |
709903 |
| 3.2.1.142 | T233A |
single nucleotide polymorphism associated with thermostability |
709903 |
