EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.113 | C334A |
Tm-value of mutant enzyme is 49.6°C compared to 55.8°C for the wild-type enzyme. Km-value is 2.4fold lower than wild-type value, turnover-number is 2.2fold lower than wild-type value |
664373 |
| 3.2.1.113 | C363A |
Tm-value of mutant enzyme is 49.6°C compared to 55.8°C for the wild-type enzyme. Km-value is 1.1fold higher than wild-type value, turnover-number is 1.1fold lower than wild-type value |
664373 |
| 3.2.1.113 | C443A |
Tm-value of mutant enzyme is 51.9°C compared to 55.8°C for the wild-type enzyme. Km-value is identical to wild-type value, turnover-number is 1.3fold lower than wild-type value |
664373 |
| 3.2.1.113 | C443D |
relative activity is less than 50% of wild-tzype activity |
664373 |
| 3.2.1.113 | C443G |
Tm-value of mutant enzyme is 50.2°C compared to 55.8°C for the wild-type enzyme |
664373 |
| 3.2.1.113 | C443L |
relative activity is less than 50% of wild-tzype activity |
664373 |
| 3.2.1.113 | C443M |
relative activity is less than 50% of wild-tzype activity |
664373 |
| 3.2.1.113 | C443S |
Tm-value of mutant enzyme is 50.0°C compared to 55.8°C for the wild-type enzyme |
664373 |
| 3.2.1.113 | C443T |
Tm-value of mutant enzyme is 52.8°C compared to 55.8°C for the wild-type enzyme |
664373 |
| 3.2.1.113 | C443V |
relative activity is less than 50% of wild-tzype activity |
664373 |
