EC Number |
Protein Variants |
Reference |
---|
3.13.1.3 | C17S |
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant |
-, 649406 |
3.13.1.3 | C26S |
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant |
-, 650387 |
3.13.1.3 | H60Q |
mutation causes an 17fold reduction in the specific activity |
669500 |
3.13.1.3 | Q65H |
increase in thermostability, decrease in catalytic activity |
-, 683271 |
3.13.1.3 | R70I |
inactive mutant mostly exists in the insoluble fraction of the cell extract |
669500 |
3.13.1.3 | R70K |
inactive mutant mostly exists in the insoluble fraction of the cell extract |
669500 |
3.13.1.3 | Y63A |
increase in catalytic activity |
-, 683271 |
3.13.1.3 | Y63F |
increase in catalytic activity, similar thermostability as wild-type |
-, 683271 |
3.13.1.3 | Y63F/Q65H |
increase in thermostability without loss of catalytic activity or affinity for substrate |
683271 |
3.13.1.3 | Y63K |
decrease in activity |
683271 |