EC Number   |
Protein Variants |
Reference |
|---|
    3.1.7.11 | D501A |
30% of wild-type activity |
751469 |
| 3.1.7.11 | more |
a truncated mutant which lacks the first 53 amino acids, shows improved production of geraniol |
751238 |
| 3.1.7.11 | more |
construction of domain swapping mutants between geraniol synthase from Perilla citriodora and linalool synthase from Perilla hirtella. Domains IV-1 and -4 are important for geraniol synthesis, and domains III-b, III-d, and IV-4 for linalool synthesis. The conformation of carbocation intermediates and their electron localization seem different between geraniol and linalool synthases. Five amino acids in domain IV-4 are indispensable for the formation of geraniol and linalool. These residues seem to be responsible for the different spatial arrangement of the amino acid at H524 in the case of geraniol synthase, while N526 is the corresponding residue in linalool synthase |
730561 |
| 3.1.7.11 | more |
construction of Ser35 and Met44 truncated GES proteins showing reduced activity compared to the wild-type enzyme |
718230 |
| 3.1.7.11 | more |
N-terminal truncation at residues S14, L28, S43and S52 all lead to an increase on geraniol production |
751469 |
| 3.1.7.11 | Y436A |
12% of wild-type activity |
751469 |
| 3.1.7.11 | Y436A/D501A |
10% of wild-type activity |
751469 |
