EC Number |
Protein Variants |
Reference |
---|
3.1.4.55 | D164A |
0.07% of wild-type activity |
721652 |
3.1.4.55 | D187A |
83% of wild-type activity |
721652 |
3.1.4.55 | D54A |
0.09% of wild-type activity |
721652 |
3.1.4.55 | D80A |
0.07% of wild-type activity |
721652 |
3.1.4.55 | D80A |
inactive with substrate bis(p-nitrophenyl)phosphate |
719851 |
3.1.4.55 | H200A |
10% of wild-type activity, 6.33fold decrease in kcat/KM with substituted methyl phenylphosphate diesters with leaving group pKa values ranging from 4 to 8.4. With bis(pnitrophenyl)phosphate as a substrate, there is a 10fold decrease in kcat/KM, primarily the result of a large increase in KM. The nickel ion-activated H200A mutant displays a bell-shaped pH dependence for kcat/KM with pKa values comparable to those of the wild-type enzyme |
721652 |
3.1.4.55 | H222A |
14% of wild-type activity |
721652 |
3.1.4.55 | H78A |
0.3% of wild-type activity |
721652 |
3.1.4.55 | T75A |
3% of wild-type activity |
721652 |