EC Number   |
Protein Variants |
Reference |
|---|
   3.1.4.52 | C406S |
site-directed mutagenesis of enzyme MtbDGC, the mutant is catalytically inactive |
716686 |
| 3.1.4.52 | C75A/C106A |
mutation promotes catalytic activity |
750416 |
| 3.1.4.52 | D183A |
site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme |
730416 |
| 3.1.4.52 | D222A |
site-directed mutagenesis, the mutant shows highly reduced catalytic activity compared to the wild-type enzyme |
730416 |
| 3.1.4.52 | D224A |
mutation in HDDDF motif, decrease in activity |
680492 |
| 3.1.4.52 | D225A |
mutation in HDDDF motif, strong decrease in activity |
680492 |
| 3.1.4.52 | D289A |
inactive |
-, 749912 |
| 3.1.4.52 | D289A |
VCA0681, mutation in active site, eliminated VCA0681 repression of vpsL-lux, biofilm formation not repressed by mutant contrary to wild-type. Expression of the active-site mutant (D289A) in a luxO D47E strain has little effect on cyclic di-3',5'-guanylate levels |
-, 698608 |
| 3.1.4.52 | D295A |
inactive |
692857 |
| 3.1.4.52 | D295A |
site-directed mutagenesis, inactive mutant |
692857 |
