EC Number   |
Protein Variants |
Reference |
|---|
   3.1.3.53 | A233S |
site-directed mutagenesis of subunit PP1cgamma |
715574 |
| 3.1.3.53 | A281G |
site-directed mutagenesis of subunit PP1cgamma |
715574 |
| 3.1.3.53 | D884A |
mutant is not cleaved by caspase-3 in apoptotic cells |
732482 |
| 3.1.3.53 | DELTA495-1030 |
using a deletion mutant it is shown that the C-terminal domain of human MYPT1(495-1030) is responsible for the binding to the N-terminal portion of myosin light meromyosin |
731315 |
| 3.1.3.53 | F38A |
site-directed mutagenesis of subunit MYPT1, the mutant subunit is unable to bind to subunit PP1cbeta |
715574 |
| 3.1.3.53 | G280A |
site-directed mutagenesis of subunit PP1cbeta, the mutation does not affect interaction with MYPT1 |
715574 |
| 3.1.3.53 | H237N/K238R |
site-directed mutagenesis of subunit PP1cgamma, the mutant subunit shows over 90% reduced ability to bind to subunit MYPT1 |
715574 |
| 3.1.3.53 | more |
construction of synthetic segments of MYPT1 (1-19) and (24-41) and MYPT1(1-19) and (24-41) peptides |
682882 |
| 3.1.3.53 | more |
deletion of the C-terminal residues of MYPT1 at codon 39, 172, 297 and 517 by site directed mutagenesis |
681839 |
| 3.1.3.53 | more |
expression of the caspase-3 cleaved form of MYPT1 that lacks the C-terminal end in HeLa cells causes the dissociation of MYPT1 from actin stress fibers |
732482 |
