EC Number |
Protein Variants |
Reference |
---|
3.1.27.8 | C225S |
site-directed mutagenesis, the mutant shows significantly reduced enzyme activity compared to the wild-type full-length enzyme |
728877 |
3.1.27.8 | C226S |
site-directed mutagenesis, the mutant shows significantly reduced enzyme activity compared to the wild-type full-length enzyme |
728877 |
3.1.27.8 | C227S |
site-directed mutagenesis, the mutant shows significantly reduced enzyme activity compared to the wild-type full-length enzyme |
728877 |
3.1.27.8 | C228S |
site-directed mutagenesis, the mutant shows significantly reduced enzyme activity compared to the wild-type full-length enzyme |
728877 |
3.1.27.8 | D52A |
site-directed mutagenesis of an active site residue, inactive mutant |
730457 |
3.1.27.8 | E100A |
site-directed mutagenesis of an active site residue, the mutant shows highly reduced activity compared to the wild-type enzyme |
730457 |
3.1.27.8 | more |
construction of truncated enzyme version representing residues Thr13-Ser250, i.e. hEndoV-SF. The mutant shows reduced enzyme activity compared to the wild-type full-length enzyme |
728877 |
3.1.27.8 | Y91A |
site-directed mutagenesis of an active site residue, the mutant shows highly reduced activity compared to the wild-type enzyme |
730457 |