   3.1.16.1 | more |
Trm2p(ctdelta76aa), lacks 76 amino acids at the C-terminus, retains nuclease activities but not the methyl transferase activity. Trm2(delta232-1920nt) mutant, contains only the first 231 nucleotides of the TRM2 gene, displays low sensitivity to methyl methane sulfonate and suppresses the methyl methane sulfonate sensitivity of rad52 mutants in trm2(delta232-1920nt)rad52 double mutants. Trm2 exo1 double mutants are synergistically more sensitive to methyl methane sulfonate and ionizing radiation than either of the single mutant. The ku80trm2(deltaD232-1920nt) double mutant displays much higher methyl methane sulfonate sensitivity than either of the single mutant alone. The trm2 (delta232-1920nt) single mutant conferrs only a very slight sensitivity to gamma irradiation, while the trm2(delta232-1920nt)exo1 double mutant exhibits 1.7-2.2fold higher sensitivity relative to trm2(delta232-1920nt) mutant |
681898 |
