EC Number   |
Protein Variants |
Reference |
|---|
   3.1.13.B1 | E203A/D233A/D235A |
site-directed mutagenesis, mutation of three active site residues, mutant rat1EDD does not show 5'-3' exoribonuclease activity, and rat1EDD/Rai1 complex does not degrade RNAs or decrease RNAPII elongation, demonstrating that RNA degradation by exonuclease activity is critical to RNAPII termination |
751722 |
| 3.1.13.B1 | E209A |
significantly reduced activity compared with the wild type protein |
-, 725397 |
| 3.1.13.B1 | H388A |
significantly reduced activity compared with the wild type protein |
-, 725397 |
| 3.1.13.B1 | H410A |
mutant enzyme has full activity |
-, 725397 |
| 3.1.13.B1 | H410V |
significant loss of activity |
725397 |
| 3.1.13.B1 | H86A |
significantly reduced activity compared with the wild type protein |
-, 725397 |
| 3.1.13.B1 | more |
to address the biological significance of Saci-aCPSF2, a deletion mutant is constructed, and the influence of SaciaCPSF2 on the transcriptome profile is assessed employing high throughput RNA sequencing |
-, 726351 |
