EC Number   |
Protein Variants |
Reference |
|---|
   3.1.11.6 | A188T |
site-directed mutagenesis of a highly conserved residue. The A188T mutant shows a msDNA cleavage indistinguishable from that of the wild-type, and lethality is reduced. About 10times more colonies are observed from the A188T mutant than from the wild-type |
751292 |
| 3.1.11.6 | D155A |
mutant of large subunit XseA, complete loss of catalytic activity |
730489 |
| 3.1.11.6 | D155N |
site-directed mutagenesis of a highly conserved residue. The D155N mutant loses the ability to cleave msDNA and displays little lethality |
751292 |
| 3.1.11.6 | D235A/D240A |
is completely inactive, even in the presence of magnesium |
700496 |
| 3.1.11.6 | D241A |
mutant of large subunit XseA, complete loss of catalytic activity |
730489 |
| 3.1.11.6 | DELTA397-456 |
deletion mutant of large subunit XseA, complete loss of catalytic activity |
730489 |
| 3.1.11.6 | F63A |
mutant of large subunit XseA, decrease in DNA-binding activity |
730489 |
| 3.1.11.6 | G237R |
site-directed mutagenesis of a residue in the glycine-rich motif. No msDNA cleavage is found for the G237R mutant but a low level of cell killing is still observed. The culture viability drops to about 30% after a 2 h induction |
751292 |
| 3.1.11.6 | H238A |
mutant of large subunit XseA, complete loss of catalytic activity |
730489 |
| 3.1.11.6 | L88R |
site-directed mutagenesis |
751292 |
