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Results 1 - 10 of 15 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2D251A site-directed mutagenesis, the mutant shows a 3fold decline in the endonucleolytic cleavage activity -, 749954
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2D277A mutant lacking all enzymatic activities 682165
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2E117D mutant is unable to cleave substrate in vitro without affecting substrate binding, substitution of Mn2+ for Mg2+ increases the activity 650060
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2E117Q mutant is unable to cleave substrate in vitro but can bind substrate, substitution of Mn2+ for Mg2+ does not increase the activity 650060
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2E39A inactive mutant enzyme ExoIII -, 752239
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2E57A site-directed mutagenesis, the mutant shows a 2fold decline in the endonucleolytic cleavage activity -, 749954
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2E57A/D251A site-directed mutagenesis, the double mutant shows no 3'-5' exonuclease activity -, 749954
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2E58A altered Mg2+-binding properties, 3'-diesterase activity is reduced when compared to the native enzyme 664070
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2F242S site-directed mutagenesis, the mutant shows slightly increased 3'-5' exonuclease catalytic efficiency compared to wild-type 749954
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.2more elucidation of the iVEC mechanism at the molecular level avances the development of in vivo DNA cloning technology. Multiple-fragment assembly of up to seven fragments in combination with an effortless transformation procedure using a modified host strain for iVEC is possible. In vitro recombination system for DNA cloning already allow the joining of multiple DNA fragments at once. Construction of an Escherichia coli strain that is optimized for in vivo cloning. Generation of exonuclease deletion mutants from strain BW25113, the parental strain of the Keio collection, has sufficient capacity for iVEC activity 750978
Results 1 - 10 of 15 > >>