EC Number |
Protein Variants |
Reference |
---|
3.1.1.64 | A132T |
50% activity compared to the wild type enzyme |
716734 |
3.1.1.64 | A434V |
55% activity compared to the wild type enzyme |
716734 |
3.1.1.64 | C106A |
the mutation does not affect the palmitylation status of the fragment 98-118, the mutant remains associated with the membrane. The mutant has an intact enzymatic activity similar to that of wild type enzyme |
715501 |
3.1.1.64 | C106Y |
site-directed mutagenesis, the mutant exhibits 16% reduced activity compared to the wild-type enzyme |
730062 |
3.1.1.64 | C106Y/K297G |
site-directed mutagenesis, the mutant exhibits 26% reduced activity compared to the wild-type enzyme |
730062 |
3.1.1.64 | C112A |
the mutation abolishes palmitylation in fragment 98-118 and dissociates RPE65 from the membrane. The mutant has no enzymatic activity |
715501 |
3.1.1.64 | C231S |
55% activity compared to the wild type enzyme |
716734 |
3.1.1.64 | C329S |
26% activity compared to the wild type enzyme |
716734 |
3.1.1.64 | C329S/C330S |
less than 2% activity compared to the wild type enzyme |
716734 |
3.1.1.64 | C330T |
83% activity compared to the wild type enzyme |
716734 |