EC Number |
Protein Variants |
Reference |
---|
3.1.1.6 | C16A |
the mutant shows strongly reduced activity (to about 10%) compared to the wild type enzyme |
750537 |
3.1.1.6 | D179A |
the mutant shows strongly reduced activity (to about 50%) compared to the wild type enzyme |
750537 |
3.1.1.6 | E188D |
very low specific activity, R enantioselectivity of above 100, S enantioselectivity of 26, mutant generated using a focused directed-evolution approach based on saturation mutagenesis according to CASTing method |
695582 |
3.1.1.6 | E188W |
very low specific activity similar to mutant M193C, S enantioselectivity of 26 |
695582 |
3.1.1.6 | E188W/M193C |
14% of wild-type specific activity, wider substrate range than wild-type with respect to tertiary alcohol acetates, inversed enantioselectivity, S enantioselectivity of 64, binding of S enantiomer of substrate 1,1,1-trifluoro-2-phenyl-but-3-yn-1-yl acetate with lower free energy than wild-type, M193C mutation seems to stabilize S orientation, mutant generated using a focused directed-evolution approach based on saturation mutagenesis according to CASTing method |
695582 |
3.1.1.6 | F152A |
the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis |
750541 |
3.1.1.6 | H182A |
the mutant shows strongly reduced activity (to about 50%) compared to the wild type enzyme |
750537 |
3.1.1.6 | L209F |
the mutant shows 3.8fold increased activity compared to the wild type enzyme |
729428 |
3.1.1.6 | L97F |
the mutant shows 5.4fold increased activity compared to the wild type enzyme |
729428 |
3.1.1.6 | L97F/L209F |
the mutant shows 28.8fold increased activity compared to the wild type enzyme |
729428 |