EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.59 | F259I |
lower affinity for 3-octylthio-1,1,1-trifluoropropan-2-one |
667723 |
| 3.1.1.59 | F259I |
the overall structure of the substrate binding pocket is similar to that of wild-type JHE. Shows a 5.7fold higher kcat for juvenile hormone III as the wild-type. KM of mutant F259I for juvenile hormone III is 3.4fold higher than that of the wild-type. With the saturated form of juvenile hormone III as a substrate, mutant F259I shows a 4.4fold higher kcat and 14fold higher Km in comparison to the wild-type |
707525 |
| 3.1.1.59 | more |
silencing of the enzyme by RNA interference, i.e. baculovirus-mediated RNAi, no specific phenotype, overview |
730748 |
| 3.1.1.59 | T314V |
no major changes in the overall structure of the substrate binding pocket. The KM of mutant T314V for juvenile hormone III is 2.9fold higher than that of JHE, whereas kcat is 6.4fold higher. Specific activity of mutant T314V for juvenile hormone III diol is not statistically different than that of wild-type for juvenile hormone III |
707525 |
