EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.52 | K271N/K273N/K274N |
wild-type activity, half the wild-type increase in activity in presence of phosphatidylinositol 4,5-biphosphate |
693262 |
| 3.1.1.52 | K43N |
impaired release of arachidonic acid in response to serum and phorbol myristate acetate, no translocation to Golgi apparatus in response to serum, C2-domain mutant, calcium and membrane binding |
693262 |
| 3.1.1.52 | K488N/K543N/K544N |
2fold increased specific activity (independent of phosphatidylinositol 4,5-biphosphate), 78% reduced release of arachidonic acid and increased Golgi-binding in response to serum, PIP2-binding site triple mutant |
693262 |
| 3.1.1.52 | K541N/K543N/K544N |
2fold increased specific activity (independent of phosphatidylinositol 4,5-biphosphate) |
693262 |
| 3.1.1.52 | S505A |
38% reduced release of arachidonic acid and prolonged accumulation at Golgi apparatus in response to serum and 30% reduced release of arachidonic acid in response to phorbol myristate acetate, phosphorylation site mutant |
693262 |
| 3.1.1.52 | S505A/S727A |
30% reduced release of arachidonic acid in response to phorbol myristate acetate and 50% in response to serum which is further reduced by ERK inhibition, prolonged accumulation at Golgi apparatus in response to serum, phosphorylation site double mutant |
693262 |
| 3.1.1.52 | S505A/S727A/S515A |
similar response to serum as double mutant S505A/S727A, phosphorylation site triple mutant |
693262 |
| 3.1.1.52 | S515A |
phosphorylation site mutant |
693262 |
| 3.1.1.52 | S727A |
similar release of arachidonic acid in response to serum like wild-type enzyme, phosphorylation site mutant |
693262 |
