EC Number   |
Protein Variants |
Reference |
|---|
    2.8.3.12 | E54A |
0.02% as active as wild-type enzyme |
645955 |
| 2.8.3.12 | E54A |
mutant without CoA-transferase or acyl-CoA hydrolase activity |
94485 |
| 2.8.3.12 | E54A |
mutation in the subunit GctB, inactive mutant |
645955, 645957, 94485 |
| 2.8.3.12 | E54D |
above 7% as active as wild-type enzyme, mutant with changed catalytic mechanism without CoASH intermediate, the Escherichia coli strain producing mutant enzyme shows a lower growth rate and reduced amount of recombinant enzyme |
645955 |
| 2.8.3.12 | E54D |
mutation decreases CoA-transferase and increases acyl-CoA hydrolase activity, mechanism, mutant catalyses the hydrolysis of glutaryl-CoA, acetyl-CoA and 3-butenoyl-CoA, mutant Escherichia coli strain shows reduced growth rate after induction |
94485 |
| 2.8.3.12 | E54D |
mutation in the subunit GctB |
645955, 645957, 94485 |
| 2.8.3.12 | E54D |
replacement of the catalytic glutamate by aspartate converts the mutant enzyme to a thiol ester hydrolase |
645957 |
| 2.8.3.12 | E54N |
mutant without CoA-transferase or acyl-CoA hydrolase activity |
94485 |
| 2.8.3.12 | E54N |
mutation in the subunit GctB, inactive mutant |
645957, 94485 |
| 2.8.3.12 | E54Q |
1% as active as wild-type enzyme, by incubating with both substrates for 20 h at room temperature, glutamine is completely converted to glutamate yielding a fully active CoA-transferase |
645957 |
