EC Number |
Protein Variants |
Reference |
---|
2.8.1.12 | E125K |
inactive mutant |
721609 |
2.8.1.12 | E128K |
inactive mutant |
721609 |
2.8.1.12 | E128K |
site-directed mutagenesis, mutant of MoaE, the MPT synthase containing E128K MoaE is 16.6times slower than the wild-type protein |
722628 |
2.8.1.12 | E141DELTA |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, 12.3times slower than the wild-type protein, truncation of the C-terminal helix might be predicted to disrupt interaction with MoaD-SH |
722628 |
2.8.1.12 | E141DELTA |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, formate binding site structure in the E141DELTA MoaE variant, overview |
722627 |
2.8.1.12 | E168K |
naturally occuring substitution in MOCS2B, the E168K mutation, identified in a severely affected patient, attenuates binding of precursor Z, the MPT synthase tetramer is readily formed in mixtures of MOCS2B-E168K with equimolar amounts of MOCS2A |
722634 |
2.8.1.12 | F34A |
site-directed mutagenesis, mutant of MoaE, the mutant shows reduced activity compared to the wild-type enzyme |
722628 |
2.8.1.12 | G81DELTA |
deletion of the MoaD C-terminal glycine (G81DELTA MoaD-SH) completely abolishes MPT synthase activity |
722628 |
2.8.1.12 | K119A |
site-directed mutagenesis, mutant of MoaE, the mutant shows loss of activity, probably due to complete failure to form the heterotetramer |
722628 |
2.8.1.12 | K123A |
site-directed mutagenesis, mutant of MoaE |
721609 |