EC Number |
Protein Variants |
Reference |
---|
2.8.1.11 | A228T |
random mutagenesis, from mutant strain F36, the chromosomal mutant of moeB demonstrates limited molybdenum cofactor molybdo-bis(molybdopterin guanine dinucleotide) availability in Escherichia coli, the moeBA228T mutation disrupts the interaction between MoeB and MoaD. The mutant is defective in anaerobic respiration and growth on DMSO |
721512 |
2.8.1.11 | C316A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme |
723621 |
2.8.1.11 | C316A/C324A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme |
721600 |
2.8.1.11 | C316A/C324A/C365A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme |
721600 |
2.8.1.11 | C324A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme |
723621 |
2.8.1.11 | C365A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme |
723621 |
2.8.1.11 | C412A |
site-directed mutagenesis, exchange of the conserved active site loop residue, inactive mutant |
721600, 723621 |
2.8.1.11 | D417R |
site-directed mutagenesis, the kcat of the mutant variant is increased 83fold when dithiothreitol is used as reductant, or 470fold when cyanide is used as sulfur acceptor |
722226 |
2.8.1.11 | D417T |
site-directed mutagenesis, the mutant shows a 17fold increased dithiothreitol:thiosulfate oxidoreductase activity or a 90fold increased thiosulfate:cyanide sulfurtransferase activity |
722226 |
2.8.1.11 | G160D |
radom mutagenesis, temperature-sensitive phenotype |
722607 |