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EC Number Protein Variants Commentary Reference
Show all pathways known for 2.8.1.10Display the word mapDisplay the reaction diagram Show all sequences 2.8.1.10D182A no residual activity 707468
Show all pathways known for 2.8.1.10Display the word mapDisplay the reaction diagram Show all sequences 2.8.1.10E98A less than 3% residual activity 707468
Show all pathways known for 2.8.1.10Display the word mapDisplay the reaction diagram Show all sequences 2.8.1.10more overexpression of His-tagged proteins ThiF, NifS, ThiO, and ThiSG Escherichia coli BL21and incubation with L-cysteine in the presence of dithiothreitol and ATP to form ThiS-COSH. This is then added to 1-deoxy-D-xylulose 5-phosphate and glycine in the presence of ThiO and ThiG to produce the product of the thiazole synthase-catalyzed reaction. Reaction product is a dearomatized thiazole tautomer 708874
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