EC Number   |
Protein Variants |
Reference |
|---|
   2.7.8.33 | D156E |
no detectable transfer activity |
708918 |
| 2.7.8.33 | D156N |
no detectable transfer activity |
708918 |
| 2.7.8.33 | D159E |
transfer activity is drastically reduced, compared to wild-type enzyme |
708918 |
| 2.7.8.33 | D159N |
transfer activity is drastically reduced, compared to wild-type enzyme |
708918 |
| 2.7.8.33 | D217A |
the mutation results in reduced enzymatic activity |
723725 |
| 2.7.8.33 | D217E |
the mutation results in a slightly more active enzyme |
723725 |
| 2.7.8.33 | D217K |
inactive |
723725 |
| 2.7.8.33 | D217N |
the mutation results in a more active enzyme and leads to more than 2fold increase in Vmax without significant change in the Km for the UDP-N-acetyl-D-glucosamine |
723725 |
| 2.7.8.33 | D217S |
the mutation results in a more active enzyme |
723725 |
| 2.7.8.33 | D72A |
site-directed mutagenesis, very low activity of the mutant protein at pH 8.0, which represents only about 1.2% of the wild-type activity. At pH 7.0, the mutant protein is totally inactive. Increasing the pH from 8 to 9 results in a 2.5fold increase of the D72A mutant activity, while the wild-type enzyme activity rather decreases, from 310 to 240 U/mg of protein |
-, 737788 |
