EC Number   |
Protein Variants |
Reference |
|---|
    2.7.8.13 | D115N |
mutatio eliminates translocase 1 activity |
662009 |
| 2.7.8.13 | D116N |
mutatio eliminates translocase 1 activity |
662009 |
| 2.7.8.13 | D174N |
0.39% of wild-type activity, 70% of optimum activity in presence of 250 mM Mg2+, altered catalytic efficiency (kcat), not capable of functional complementation, invariant residue in third cytoplasmic segment |
690996 |
| 2.7.8.13 | D177N |
1.6% of wild-type activity, 70% of optimum activity in presence of 250 mM Mg2+, altered catalytic efficiency (kcat), invariant residue in third cytoplasmic segment |
690996 |
| 2.7.8.13 | D231N |
0.42% of wild-type activity, altered catalytic efficiency (kcat), not capable of functional complementation, invariant residue in fourth cytoplasmic segment |
690996 |
| 2.7.8.13 | D231N |
catalytically inactive, overexpression does not rescue bacteria from protein E-induced lysis |
692509 |
| 2.7.8.13 | D267N |
enzymatically inactive, overexpression rescues bacteria from protein E-induced lysis |
692509 |
| 2.7.8.13 | D267N |
mutatio eliminates translocase 1 activity |
662009 |
| 2.7.8.13 | D98A |
site-directed mutagenesis, the mutant shows altered pH dependence compared to the wild-type enzyme |
-, 737788 |
| 2.7.8.13 | D98N |
0.014% of wild-type activity, shifted pH optimum (pH 9.0-9.4) compared to wild-type (pH 7.5), highly decreased turnover (kcat, pH 7.2, elevated turnover at pH 9.4), decreased KM for UDP-N-acetylmuramoyl-pentapeptide (pH 7.2 and pH 9.4), increased KM for undecaprenyl phosphate (2fold at pH 7.2, 6fold at pH 9.4), not capable of functional complementation, invariant residue in second cytoplasmic segment |
690996 |
