EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.85 | A61V |
11.6fold increase in cyclic di-AMP production |
-, 738619 |
| 2.7.7.85 | A76V |
4.3fold increase in cyclic di-AMP production |
-, 738619 |
| 2.7.7.85 | D75N |
inactive |
-, 737649 |
| 2.7.7.85 | E46K |
19.5fold increase in cyclic di-AMP production |
-, 738619 |
| 2.7.7.85 | G206S |
single nucleotide polymorphism identified in in a faster growing but less resistant strain of an isogenic MRSA strain pair that differs in methicillin resistance levels and fitness |
-, 739477 |
| 2.7.7.85 | L44F |
90.5fold increase in cyclic di-AMP production |
-, 738619 |
| 2.7.7.85 | more |
both N-terminally truncated protein lacking first 100 residues, i.e. the transmembrane domain and the CC motif, and the N-terminally truncated protein lacking first 80 residues are enzymatically active, The shorter DAC variant is 6.5fold more active than the longer variant |
-, 738669 |
| 2.7.7.85 | more |
deletion of the N-terminal YojJ domain leads to complete loss of activity. Truncated proteins CdaS28-201, CdaS17-201, and CdaS11-201 show no diadenylate cyclase activity and CdaS10-201, CdaS9-201, CdaS8-201, CdaS7-201, and CdaS5-201 have only weak activity, while CdaS4-201 activity is comparable with wild type CdaS |
-, 738291 |
| 2.7.7.85 | more |
generation of the DELTA300cdaA allele, which lacks the transmembrane domain |
-, 761490 |
| 2.7.7.85 | more |
transformation and construction of deletion mutants in Haloferax volcanii strain H26 based on selection with uracil, gene dacZ knockout and overexpression. Because neither gene deletion nor overexpression of dacZ had been possible, a promotor exchange approach is used to examine the effects of altered intracellular c-di-AMP levels. Two mutant strains are created where the native promotor of dacZ is replaced with the tryptophan-inducible promotor (p.tnaA) of the tryptophanase gene tnaA (HVO_0789) or, respectively, with a genetically engineered version of p.tnaA with about 50% reduced activity (with regard to p.tnaA) (p.tnaM3). In comparison to the wild type, the p.tnaA-dacZ strain exhibits in general slightly elevated OD600 values in this condition The p.tnaM3-induced mutant has a different phenotype as it exhibits a prolonged lag phase followed by an extended exponential phase that peaked at an increased maximal OD. Changed intracellular c-di-AMP levels causes increased cell volume in medium with low sodium chloride concentration, phenotypes, overview. When the expression of DacZ is reduced, it leads in turn to decreased c-di-AMP concentrations within the two mutant strains which leads to ta hypo-salt phenotype |
-, 761855 |
