EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.42 | D173N/D175N |
inactivation of the N-terminal nucleotidyltransferase domain by mutation of both highly conserved aspartates, mutated enzyme shows adenylyltransferase activity but lacks adenylyl-removing activity |
672242 |
| 2.7.7.42 | D438Y/R657S/S684K |
temperature-sensitive mutant |
-, 734070 |
| 2.7.7.42 | D463N/P467A/L469G |
clustered point mutations in the central region of ATase, only 30-50% adenylyl-removing and adenylyltransferase activity of wild type enzyme, adenylyl-removing activity is inhibited by signal transduction protein PII and glutamine, adenylyltransferase activity is regulated by glutamine, signal transduction protein PII and signal transduction protein PII-UMP |
672242 |
| 2.7.7.42 | D701N/D703N |
inactivation of the C-terminal nucleotidyltransferase domain by mutation of both highly conserved aspartates, mutated enzyme shows adenylyl-removing activity but lacks adenylyltransferase activity |
672242 |
| 2.7.7.42 | D720A |
mutation in the adenylylation domain, compromises activity. Strain shows reduced growth rates in the low-ammonia- and glutamine-containing media |
-, 734070 |
| 2.7.7.42 | D732A |
mutation in the adenylylation domain, mutation does not completely abrogate the enzyme activity |
-, 734070 |
| 2.7.7.42 | delata456-577 |
enzyme missing amino acids 456-577 from the central region of ATase lacks adenylyl-removing activity but retains adenylyltransferase activity, adenylyltransferase activity is inhibited by signal transduction protein PII and signal transduction protein PII-UMP |
672242 |
| 2.7.7.42 | L525G/R527A/I528G |
clustered point mutations in the central region of ATase, enzyme completely lacks adenylyl-removing activity but retains adenylyltransferase activity, adenylyltransferase activity is strongly enhanced by glutamine |
672242 |
| 2.7.7.42 | more |
all C-terminal truncation constructs are dependent on Gln for full adenylylation activity |
673595 |
| 2.7.7.42 | more |
construction of null mutant LNDELTAglnE strain with chromosomal deletion of gene glnE, the mutant does not respond to nitrogen level like the wild-type, the mutant shows a higher [L-glutamate:ammonia ligase (ADP-forming)] activity level compared to the wild-type when grown in nitrogen-rich medium due to upregulation of glnA |
643336 |
