EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.101 | E175G |
complete loss of activity |
-, 751322 |
| 2.7.7.101 | E175Q |
mutation drastically reduces priming activity, low level of DNA binding activity |
720237 |
| 2.7.7.101 | E175Q |
site-directed mutagenesis of the TOPRIM domain, DnaGE175Q has a poly(rA) preference like wild-type DnaG |
739191 |
| 2.7.7.101 | F615A |
site-directed mutagenesis, mutant MtDnaG-CTD F615A |
-, 760530 |
| 2.7.7.101 | I605A |
site-directed mutagenesis, mutant MtDnaG-CTD I605A, binding study shows a significant decrease in the binding affinity of MtDnaB-NTD with the Ile605Ala mutant of MtDnaG-CTD compared with native MtDnaG-CTD |
-, 760530 |
| 2.7.7.101 | K232A |
more than 50% decrease in DNA binding activity |
-, 752228 |
| 2.7.7.101 | K447A |
interaction of mutant with SSB is severely impaired |
751718 |
| 2.7.7.101 | K447A |
site-directed mutagenesis, the DnaGC point mutant shows dramatically attenuated SSB-Ct binding |
760348 |
| 2.7.7.101 | K478A |
mutant shows binding affinities to SSB similar to wild-type DnaG C-terminus |
751718 |
| 2.7.7.101 | K518A |
interaction of mutant with SSB is severely impaired |
751718 |
