EC Number |
Protein Variants |
Reference |
---|
2.7.1.59 | C131S |
strongly decreased activity, very low affinity for N-acetyl-D-glucosamine |
641703 |
2.7.1.59 | C143S |
strongly decreased activity, very low affinity for ATP |
641703 |
2.7.1.59 | C143S |
the mutant retains 75% of wild type enzymatic activity |
739071 |
2.7.1.59 | C211S |
slightly decreased activity |
641703 |
2.7.1.59 | C217S |
decreased activity, low affinity for N-acetyl-D-glucosamine |
641703 |
2.7.1.59 | C268S |
decreased activity |
641703 |
2.7.1.59 | C45S |
very slightly decreased activity, no function in substrate binding |
641703 |
2.7.1.59 | D107A |
inactive |
739071, 759020 |
2.7.1.59 | more |
construction of a homozygous hxk1 mutant H8-1-103 |
723600 |
2.7.1.59 | more |
deletion mutants lack enzyme activity in the cytoplasm, but do not accumulate N-acetyl-D-glucosamine suggesting the existence of an alternative pathway for reutilization of N-acetyl-D-glucosamine, overview |
659055 |