EC Number   |
Protein Variants |
Reference |
|---|
   2.7.1.197 | C384D |
inactive |
736389 |
| 2.7.1.197 | C384H |
inactive |
736389, 737220 |
| 2.7.1.197 | C384S |
active site mutant of enzyme domain B |
762334 |
| 2.7.1.197 | C395S |
active site mutant of enzyme domain B |
762334 |
| 2.7.1.197 | E218A |
the mutant has a high Km for mannitol but can carry out facilitated diffusion of mannitol at a rate higher than the wild type protein in its unphosphorylated form |
736381 |
| 2.7.1.197 | E257A |
the mutant does not detectably bind mannitol up to a concentration of at least 1 mM |
736381 |
| 2.7.1.197 | G253E |
the mutant cannot transport but still can phosphorylate mannitol |
736381 |
| 2.7.1.197 | H156A |
the mutant shows wild type activity |
736389 |
| 2.7.1.197 | H195A |
the mutant has a 40fold higher KD for mannitol compared to the wild type protein and is defective in mannitol phosphorylation even at high mannitol concentrations |
736381 |
| 2.7.1.197 | H195A |
the mutant shows very low activity compared to the wild type enzyme |
736389 |
