EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.48 | D45A |
decrease in kcat and increase in Km value of succinyl-L-homoserine substrate |
723726 |
| 2.5.1.48 | D45F |
reduction in catalytic effciency, without change in reaction specificity |
702106 |
| 2.5.1.48 | D45F/E325Y |
reduction in catalytic effciency, without change in reaction specificity |
702106 |
| 2.5.1.48 | D45N |
decrease in kcat and increase in Km value of succinyl-L-homoserine substrate |
723726 |
| 2.5.1.48 | E325A |
decrease in kcat and increase in Km values of substrates |
723726 |
| 2.5.1.48 | E325Q |
kinetic values similar to wild-type |
723726 |
| 2.5.1.48 | E325Y |
reduction in catalytic effciency, without change in reaction specificity |
702106 |
| 2.5.1.48 | F97Y |
enzymatic activity at 80°C is similar to wild-type at acidic to neutral pH, and about 50% of wild-type at basic pH |
-, 758595 |
| 2.5.1.48 | F97Y |
the mutant shows reduced activity compared to the wild type enzyme |
-, 758595 |
| 2.5.1.48 | more |
co-expression of Arabidopsis thaliana cystathionine gamma-synthase and bacterial feedback-insensitive aspartate kinase in tobacco. Plants co-expressing both enzymes have significantly higher methionine and threonine levels compared with the levels found in wild-type plants, but the methionine level does not increase beyond that found in plants expressing cystathionine gamma-synthase alone. Plants expressing bacterial feedback-insensitive aspartate kinase and one of two mutated forms of cystathionine gamma-synthase in which the domains responsible for the feedback regulation have been deleted, show significantly higher methionine contents and its metabolites levels accumulate in the newly produced plants. The levels of threonine are also significantly higher than in the wild-type plants. The transcript level of the two mutated forms of cystathionine gamma-synthase significantly increases when there is a high content of threonine in the plants, suggesting that threonine modulates, probably indirectly, the transcript level of cystathionine gamma-synthase |
689547 |
