EC Number   |
Protein Variants |
Reference |
|---|
   2.4.2.43 | C148A/C149A/C173S/C216S/C318S/C383S/C400S/C411S |
when assayed for growth in the presence of polymyxin, the cysteine-free construct of ArnT supports growth at a level similar to that of the native protein |
702372 |
| 2.4.2.43 | C148S/C149S/C173S/C216S/C318S/C383S/C400S/C411S |
mutant enzyme is inactive |
702372 |
| 2.4.2.43 | C173S/C216S/C318S/C383S/C400S/C411S |
when assayed for growth in the presence of polymyxin, the cysteine-free construct of ArnT supports growth at 70% of the native protein |
702372 |
| 2.4.2.43 | D39A |
inactive |
738342 |
| 2.4.2.43 | E40A |
inactive |
738342 |
| 2.4.2.43 | E493C |
inactive |
739657 |
| 2.4.2.43 | E84A |
inactive |
-, 739708 |
| 2.4.2.43 | K69C |
inactive |
739657 |
| 2.4.2.43 | more |
analysis of 31 point mutations within a putative periplasmic loop of the cysteine-free ArnT protein, carried out using an in vivo growth assay coupled with expression studies, identification of the first time specific critical residues within the bacterial transferase. These critical residues fall into two categories: those that disrupt initial protein folding or membrane localization and those that fail to confer in vivo resistance to polymyxin despite being expressed to the inner membrane |
702372 |
| 2.4.2.43 | R254C |
inactive |
739657 |
