EC Number   |
Protein Variants |
Reference |
|---|
   2.4.2.38 | D550A |
mutation of first DXD motif to AXD, complete loss of activity, expression in Saccharomyces cerevisiae |
692993 |
| 2.4.2.38 | D659A |
mutation of second DXD motif to AXD, dramatic reduction in activity, expression in Saccharomyces cerevisiae |
692993 |
| 2.4.2.38 | more |
co-expression of human monoclonal antibodies with specific RNAi to block beta-1,2-xylosyltransferase and alpha-1,3-fucosyltransferase prevents unwanted plant specific N-glycosylation of the recombinant antibody proteins, method optimization, overview |
676122 |
| 2.4.2.38 | more |
construction of several N-terminal deletion mutants, the first 54 residues can be deleted without affecting the catalytic activity of the enzyme, removal of an additional five amino acids lead to the formation of an inactive protein |
671831 |
| 2.4.2.38 | more |
construction of transgenic Nicotiana benthamiana plants expressing the CTSregion, T and C region, and a membrane-shed enzyme version, the latter is secreted to the apoplast, the T or C region constructs are located in the cytoplasm, the CTS construct is located in the cytoplasmic membrane, overview |
660192 |
| 2.4.2.38 | more |
serial deletions at both the N- and the C-terminal ends, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, construction of transgenic tobacco expressing the enzyme via Agrobacterium tumefaciens infection system |
660169 |
| 2.4.2.38 | more |
targeted downregulation of the enzyme and the alpha1,3-fucosyltransferase allow production of heterologous proteins in a maize expression system |
676375 |
| 2.4.2.38 | N141Q |
mutation of the N-glycosylation site, molecular weight shift to 79 kDa corresponding to the molecular weight of the unmodified enzyme, no change in activity, expression in Saccharomyces cerevisiae |
692993 |
| 2.4.2.38 | S53A |
site-directed mutagenesis, possesses the N-glycosylation sites Asn301 and Asn478 |
660169 |
| 2.4.2.38 | S53A/T303A |
site-directed mutagenesis |
660169 |
