EC Number |
Protein Variants |
Reference |
---|
2.4.2.21 | C160A |
loss of catalytic efficiency by 79%. Renders the enzyme 5fold less efficient than wild-type. Displays no discernible growth defect in no-carbon essential (NCE) medium containing dicyanocobinamide and glycerol or ethanolamine relative to that of a strain that synthesizes wild-type |
704323 |
2.4.2.21 | G171D |
loss of catalytic efficiency by more than 99%, has less affinity for 5,6-dimethylbenzimidazole. Retains activity to support growth, but only when 5,6-dimethylbenzimidazole is present in the medium. Does not support the growth of strain JE2423 on ethanolamine in medium that contains dicyanocobinamide but lacks 5,6-dimethylbenzimidazole |
704323 |
2.4.2.21 | G171D/M325A |
requires cobalamin for growth regardless of the carbon source used |
704323 |
2.4.2.21 | G257A |
loss of catalytic efficiency by 64% |
704323 |
2.4.2.21 | G257D |
loss of catalytic efficiency by more than 99%, has less affinity for 5,6-dimethylbenzimidazole. Does not respond to 5,6-dimethylbenzimidazole and requires cobalamin to grow. Does not support the growth of strain JE2423 on ethanolamine in medium that contains dicyanocobinamide but lacks 5,6-dimethylbenzimidazole |
704323 |
2.4.2.21 | G257D/P12G |
is cobalamin auxotroph on glycerol or ethanolamine. P12G does not alter the effect of the G257D mutation |
704323 |
2.4.2.21 | G320D |
loss of catalytic efficiency by more than 99%, is unstable, retains activity to support growth, but only when 5,6-dimethylbenzimidazole is present in the medium. Does not support the growth of strain JE2423 on ethanolamine in medium that contains dicyanocobinamide but lacks 5,6-dimethylbenzimidazole |
704323 |
2.4.2.21 | H289A |
counteracts the effect of the G320D substitution |
704323 |
2.4.2.21 | I321S |
specific activity with p-cresol and 5,6-dimethylbenzimidazole decreased |
721677 |
2.4.2.21 | M325A |
grows in the absence of exogenous 5,6-dimethylbenzimidazole, albeit at a rate slower than that of a strain making wild-type. Addition of 5,6-dimethylbenzimidazole to the medium allows the strain making M325A to grow as fast as a strain making wild-type |
704323 |