EC Number   |
Protein Variants |
Reference |
|---|
    2.4.2.18 | D223N |
kcat is 5.5fold higher than wild-type value at 2 mM Mg2+ |
680670 |
| 2.4.2.18 | D282A |
inactive |
735669 |
| 2.4.2.18 | D282A |
mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) |
721132 |
| 2.4.2.18 | D83G |
inhibition by Mg2+ only at very high concentrations, alters the binding mode of the substrate Mg2+-5-phospho-alpha-D-ribose 1-diphosphate |
702308 |
| 2.4.2.18 | D83G/F149S |
activity increases up to about 2-5 mM MgCl2 and decreases only moderately at higher Mg2+ concentrations |
702308 |
| 2.4.2.18 | D83G/F149S |
mutation leads to monomerization, apparent melting temperature is 9.5°C lower than the wild-type value |
-, 724752 |
| 2.4.2.18 | E224Q |
kcat is 5fold higher than wild-type value at 2 mM Mg2+ |
680670 |
| 2.4.2.18 | F149S |
is inhibited by MgCl2 to a similar extent as wild-type, facilitates product release by increasing the conformational flexibility of the enzyme |
702308 |
| 2.4.2.18 | G308L |
inactive |
735669 |
| 2.4.2.18 | G308L |
mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) |
721132 |
