EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.99 | C203N |
the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme |
700775 |
| 2.4.1.99 | L322F |
the mutant shows wild type activity |
700775 |
| 2.4.1.99 | M173L |
the mutant shows wild type activity |
700775 |
| 2.4.1.99 | more |
chimeric enzymes with the large subunit of festuca 1-SST (LSuB) and the small subunit of barley sucrose:fructan 6-D-fructosyltransferase have the same catalytic specificity as the native festuca 1-SST |
673623 |
| 2.4.1.99 | more |
chimeric enzymes with the large subunit of Festuca enzyme and the small subunit of barley sucrose:fructan 6-D-fructosyltransferase have the same catalytic specificity as the native festuca enzyme and vice versa |
658740 |
| 2.4.1.99 | more |
transformation of invertase EC 3.2.1.26 into sucrose:sucrose 1-fructosyltransferase by disruption of hydrogen bonding network by site directed mutagenesis in the 23WMNDPNG29 motif. Invertase mutant W23Y shows moderate increase in 1-kestose production, such as invertase mutant N25S. Double mutant W23Y/N25S shows a 17fold increase in transfructosylation capacity |
689243 |
| 2.4.1.99 | W362Y |
the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme |
700775 |
| 2.4.1.99 | Y248V |
the mutant shows no activity |
700775 |
| 2.4.1.99 | Y317W |
the mutant shows wild type activity |
700775 |
| 2.4.1.99 | Y408W |
the mutant shows low activity |
700775 |
