EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.9 | A417N |
site-directed mutagenesis, unaltered activity compared to the wild-type enzyme |
719470 |
| 2.4.1.9 | A425P |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
-, 719470 |
| 2.4.1.9 | A425P/A538S/N543S/D548R/W551T |
site-directed mutagenesis, the mutant shows 65% reduced activity compared to the wild-type enzyme |
719470 |
| 2.4.1.9 | A489G |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
719470 |
| 2.4.1.9 | A538S |
site-directed mutagenesis, the mutation, located behind the general acid/base, increases the enzyme activity two to threefold |
719470 |
| 2.4.1.9 | D272N |
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme |
-, 658731 |
| 2.4.1.9 | D424N |
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme |
-, 658731 |
| 2.4.1.9 | D479A |
the mutant shows 80% of wild type transglycosylation activity. For hydrolytic activity, the catalytic turnover rate (kcat) of the mutant is slightly lower than that of the wild type enzyme |
-, 756346 |
| 2.4.1.9 | D520A |
site-directed mutagenesis, reduced activity in absence of Ca2+ at 30°C, reduction in affinity for Ca2+ at higher temperatures, overview |
-, 658750 |
| 2.4.1.9 | D520A |
the mutation causes a strong reduction of activity compared to the wild type enzyme |
-, 756524 |
