EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.8 | E487A |
mutant loses both the phosphorolytic and the synthetic activity |
-, 679537 |
| 2.4.1.8 | E487Q |
mutant loses the phosphorolytic activity, but retains the synthetic activity (13% of the activity of wild-type MapA) |
-, 679537 |
| 2.4.1.8 | more |
development of a conductometric specific phosphate biosensor based on tri-layer maltose phosphorylase composite films, recombinant enzyme from Escherichia coli is immobilized on a planar interdigitated electrode by cross-linking with saturated glutaraldehyde vapour in the presence of bovine serum albumin, method development and evaluation, overview |
690422 |
| 2.4.1.8 | more |
enzymatic synthesis of alpha-anomer-selective D-glucosides using maltose phosphorylase |
691362 |
| 2.4.1.8 | more |
Replacing maltose hydrolysis with phosphorolysis, by co-expression of the maltose phosphorylase with MAL11 maltose-transporter gene and Lactococcus lactis beta-phosphoglucomutase, in a Saccharomyces cerevisiae straain IMK289, a maltose transporter and maltase knockout strain, increases the anaerobic biomass yield on maltose in anaerobic maltose-limited chemostat cultures by 26%, thus demonstrating the potential of phosphorolysis to improve the free-energy conservation of disaccharide metabolism in industrial microorganisms, phenotype, overview |
720370 |
| 2.4.1.8 | more |
the (alpha/alpha)6-barrel loop 3 is a target for specificity engineering to achieve formation of trehalose and kojibiose by the enzyme in yields superior of maltose by reverse phosphorolysis with (alpha1, alpha1)- and alpha-(1,2)-regioselectivity, respectively. Substitution of LaMP His413-Glu421, His413-Ile418 and His413-Glu415 from loop 3, that include His413 and Glu415 presumably recognising the alpha-anomeric O-1 group of the glucose moiety at subsite 11, by corresponding segments from Ser426-Ala431 in trehalose phosphorylase and Thr419-Phe427 in kojibiose phosphorylase, thus confers LaMP with phosphorolytic activity towards trehalose and kojibiose, respectively. Substrate specificity of LaMP loop 3 variants in phosphorolysis, overview |
-, 720962 |
| 2.4.1.8 | S634V |
mutant loses both the phosphorolytic and the synthetic activity |
-, 679537 |
| 2.4.1.8 | S635V |
mutant loses both the phosphorolytic and the synthetic activity |
-, 679537 |
| 2.4.1.8 | Y351F |
mutant loses both the phosphorolytic and the synthetic activity |
-, 679537 |
