EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.227 | E125A |
amino acid substitution for evaluation of its role in the active site of MurG |
691120 |
| 2.4.1.227 | E268A |
amino acid substitution for evaluation of its role in the active site of MurG |
691120 |
| 2.4.1.227 | E268A |
site-directed mutagenesis, in contrast to wild-type MurG, the MurG lipid interaction mutant often localizes to a single pole |
722525 |
| 2.4.1.227 | F77E |
site-directed mutagenesis, the mutant shows unaltered localization and cell growth comared to wild-type. Lipid II synthesis might be totally abrogated in mutant F77E explaining why the mutant is blocked in septation, cell wall synthesis is necessary for engulfment |
735983 |
| 2.4.1.227 | H124A |
amino acid substitution for evaluation of its role in the active site of MurG |
691120 |
| 2.4.1.227 | H18A |
amino acid substitution for evaluation of its role in the active site of MurG |
691120 |
| 2.4.1.227 | L79E/F82E |
site-directed mutagenesis, the mutant does not bind anionic phospholipids but can localize to the cell poles |
722525 |
| 2.4.1.227 | more |
none of the point mutations shows lethality and cells expressing MurG-GFP point mutants grow normally in absence of the wild-type murG allele |
735983 |
| 2.4.1.227 | N127A |
amino acid substitution for evaluation of its role in the active site of MurG |
691120 |
| 2.4.1.227 | N134A |
amino acid substitution for evaluation of its role in the active site of MurG |
691120 |
