EC Number   |
Protein Variants |
Reference |
|---|
    2.4.1.226 | D184A |
mutant protein which lacks any glycosyltransferase activity but interacts with other ChSy family members shows that the glycosyltransferase activity of CSGlcA-T plays an important role in chondroitin polymerization |
690806 |
| 2.4.1.226 | F362S |
naturally occuring missense mutation of the enzyme activity-related, highly conserved residue, the mutant protein has a 56% decrease in GalNAcT-II activity and a 55% decrease in GlcAT-II activity compared to the wild-type |
735696 |
| 2.4.1.226 | more |
adenoviral gene delivery of the enzyme into intervertebral discs of 4-month -old ICR mice displays a substantial increase in the level of chondroitin sulfate biosynthesis |
674881 |
| 2.4.1.226 | more |
both CHSY1 expression and CS56 staining are dramatically decreased in CHSY1-silenced cells |
759875 |
| 2.4.1.226 | more |
deletion of the ChGn-2 gene significantly reduces LDL retention in the DIT mouse model. Chondroitin sulfate N-acetylgalactosaminyltransferase-2 deletion alleviates lipoprotein retention in early atherosclerosis and attenuates aortic smooth muscle cell migration. A functional assay of ASMCs prepared from ChGn-2-/- mice displays abrogation of platelet-derived growth factor (PDGF)-mediated migration via reduced PDGF receptor phosphorylation. Evaluation of LDL retention in a diffuse intimal thickening (DIT) model using partial carotid ligation on ChGn-2/LDL receptor double knockout (ChGn-2-/-/LDLr-/-) mice |
-, 758734 |
| 2.4.1.226 | more |
gene silencing via shRNA sequences targeting Rattus norvegicus Smad2, Smad3, CHSY1, c-jun and Sp1, the shRNAs are transfected using a lentiviral vector |
735915 |
| 2.4.1.226 | more |
generation of chondroitin sulfate (CS) N-acetylgalactosaminyltransferase-1 (T1) gene knockout (KO) mice, Ehlers-Danlos syndrome (EDS)-like phenotype and phenotypic features, histological analysis, detailed overview. The protein levels of collagen type 1 and Wnt3a are significantly decreased by T1 gene knockdown by T1KD. Downregulation of collagen type 1 and Wnt3a by T1 gene knockdown in vitro and in vivo |
760165 |
| 2.4.1.226 | more |
overexpression of CSGlcA-T increases the amount of chondroitin sulfate in HeLa cells, whereas the RNA interference of CSGlcA-T results in a reduction of the amount of chondroitin sulphate in the cells |
690806 |
| 2.4.1.226 | more |
overexpression of CSS3 increases the amount of chondroitin sulfate in HeLa cells, while the RNA interference of CSS3 results in a reduction in the amount of CS in the cells |
690806 |
| 2.4.1.226 | more |
overexpression of the enzyme in chondrocytic cells further enhances chondroitin sulfate biosynthesis but not that of the aggrecan core protein, indicating that the enzyme activity is not saturated in the cells and that aggrecan synthesized in the overexpressing cells is heavier than the native molecule |
674881 |
