EC Number |
Protein Variants |
Reference |
---|
2.3.2.B14 | C354A |
inactive |
721067 |
2.3.2.B14 | C354A |
mutant does not display adduct formation with inhibitors faropenem or 6-aminopenicillanic acid |
-, 756702 |
2.3.2.B14 | C354S |
no acylation by beta-lactams is observed |
755793 |
2.3.2.B14 | D353A |
meropenem preferentially acylates the mutant in a competition experiment |
755695 |
2.3.2.B14 | D353N |
meropenem preferentially acylates the mutant in a competition experiment |
755695 |
2.3.2.B14 | D353W |
meropenem preferentially acylates the mutant in a competition experiment. Substitution nearly abolishes acylation by biapenem but does not affect acylation by faropenem, doripenem, or meropenem |
755695 |
2.3.2.B14 | H336A |
no cysteine acylation by inhibitors can be detected in a 0-500-s time frame |
-, 756702 |
2.3.2.B14 | H352A |
mutation does not show a decrease in the rate of the acylation reaction by inhibitors |
-, 756702 |