EC Number |
Protein Variants |
Reference |
---|
2.3.1.B25 | G197A |
site-directed mutagenesis, the mutant enzyme shows altered activity compared to the wild-type enzyme producing heptaketides |
722089 |
2.3.1.B25 | G197T |
site-directed mutagenesis, the mutant enzyme shows altered activity compared to the wild-type enzyme producing hexaketides |
722089 |
2.3.1.B25 | G197W |
site-directed mutagenesis, the mutant enzyme shows altered activity compared to the wild-type enzyme producing tri- and pentaketides |
722089 |
2.3.1.B25 | G207A |
site-directed mutagenesis, the G207A mutant loses the octaketide-forming activity and yields the heptaketide aloesone in addition to 2,7-dihydroxy-5-[(4-hydroxy-2-oxo-2H-pyran-6-yl)methyl]-2-methyl-2,3-dihydro-4H-chromen-4-one/2,7-dihydroxy-5-[(4-hydroxy-2-oxo-2H-pyran-6-yl)methyl]-5-methyl-2,3-dihydro-4H-chromen-4-one |
722034 |
2.3.1.B25 | G207F |
site-directed mutagenesis, the bulky substitution G207F mutant loses the octaketide-forming activity and yields the pentaketide 2,7-dihydroxy-5-methylchromone |
722034 |
2.3.1.B25 | G207L |
site-directed mutagenesis, the bulky substitution G207F mutant loses the octaketide-forming activity and yields the pentaketide 2,7-dihydroxy-5-methylchromone |
722034 |
2.3.1.B25 | G207M |
site-directed mutagenesis, OKS G207M mutant completely loses the octaketide-forming activity, but efficiently produces an unnatural pentaketide, 2,7-dihydroxy-5-methylchromone, from five molecules of malonyl-CoA, not a 5,7-diydroxy-5-methylchromne like the pentaketide chromone synthase, EC 2.3.1.216 |
722034 |
2.3.1.B25 | G207T |
site-directed mutagenesis, the G207T mutant loses the octaketide-forming activity and yields a hexaketide, 6-(2,4-dihydroxy-6-methylphenyl)-4-methoxy-2-pyrone, from six molecules of malonyl-CoA |
722034 |
2.3.1.B25 | G207W |
site-directed mutagenesis, the mutant loses the octaketide-forming activity and yields a tetraketide, tetracetic acid lactone, along with the triketide, triacetic acid lactone, without the formation of an aromatic ring system |
722034 |
2.3.1.B25 | more |
biosynthesis of the C-glucosylated anthraquinone, dcII, a precursor for carminic acid, using a combination of enzymes derived from Aloe arborescens, Streptomyces sp. R1128, and the insect Dactylopius coccus. The pathway, which consists of AaOKS, StZhuI, StZhuJ, and DcUGT2, presents an alternative biosynthetic approach for the production of polyketides by using a type III polyketide synthase (PKS) and tailoring enzymes originating from a type II PKS system. Transient expression in Nicotiana benthamiana. Method, detailed overview |
756452 |