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EC Number
Protein Variants
Commentary
Reference
2.3.1.38
C159A
no activity
486945
2.3.1.38
C59S
no activity
486945
2.3.1.38
C92S
no remaining activity acetyl-CoA:acyl carrier protein transacylase activity, but remaining strongly reduced thiolase activity
486875
2.3.1.38
R606A
oligonucleotide-directed in vitro mutagenesis, 6.6fold increase in activity about 16000fold increase in selectivity for acetyl-CoA against malonyl-CoA, increased production of medium-chain length fatty acids
486873
2.3.1.38
R606K
oligonucleotide-directed in vitro mutagenesis, 1.7fold increase in activity about 16fold increase in selectivity for acetyl-CoA against malonyl-CoA
486873
2.3.1.38
S581A
nearly no remaining activity, below 0.03% compared to wild-type
486874
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