EC Number |
Protein Variants |
Reference |
---|
2.3.1.250 | C187A |
site-directed mutagenesis, increase in Wnt signaling activity of about 1.5fold upon expression of PORCNC187A compared to wild-type PORCN |
734745 |
2.3.1.250 | C375X |
site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 residue, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant |
734745 |
2.3.1.250 | C381X |
site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 residue, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant |
734745 |
2.3.1.250 | C385X |
site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 residue, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant |
734745 |
2.3.1.250 | H341A |
site-directed mutagenesis, catalytic active site mutant, Wnt signaling inactive mutant |
734194 |
2.3.1.250 | H341A |
site-directed mutagenesis, catalytically inactive mutant |
734745 |
2.3.1.250 | H341A |
site-directed mutagenesis, the mutation reduces Wnt signaling relative to wild-type PORCN |
734745 |
2.3.1.250 | H341D |
site-directed mutagenesis, catalytically inactive mutant |
733699 |
2.3.1.250 | H341Q |
site-directed mutagenesis, catalytically inactive mutant |
733699 |
2.3.1.250 | more |
generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview |
733769 |