EC Number |
Protein Variants |
Reference |
---|
2.3.1.230 | C112A |
binding behavior towards inhibitors is different from wild-type enzyme |
722927 |
2.3.1.230 | C112A |
inactive |
736669 |
2.3.1.230 | C112A |
the C112A mutant is inactive although it still reversibly binds anthraniloyl-CoA |
721616 |
2.3.1.230 | C112S |
about 20% of wild-type activity |
736669 |
2.3.1.230 | C112S |
about 8% of wild-type activtiy |
735348 |
2.3.1.230 | H257F |
binding behavior towards inhibitors is different from wild-type enzyme |
722927 |
2.3.1.230 | H257F |
inactive |
735348, 736669 |
2.3.1.230 | N287A |
inactive, anthraniloyl remains covalently bound to C112, further supporting that N87 does not take part in the cleavage of anthraniloyl-CoA |
736669 |
2.3.1.230 | S317A |
activity similar to wild-type |
735348 |
2.3.1.230 | S317F |
binding behavior towards inhibitors is different from wild-type enzyme |
722927 |